SmartGlow™ LD (Loading Dye) is supplied at 6X concentration and is added directly to the samples. No dye needs to be added to the gel or running buffer. After electrophoretic separation, view and document your results using a UV or blue light illuminator. SmartGlow™ LD emits green fluorescence when bound to dsDNA, ssDNA and RNA.
Protocol for SmartGlow LD
Prepare agarose for polyacrylamide solution.
Pour gels.
Mix SmartGlow LD with samples and DNA markers at a 1:5 (dye to sample) dilution.
After electrophoresis is complete, view the gel using a UV or blue light illuminator
